GRAS family proteins are one of the most abundant transcription factors in plants. They play a crucial role in plant development, their metabolism and their responses to biotic and abiotic stress. The GRAS family, The PEBP gene family is crucial for plant growth and development, the transition between vegetative and reproductive growth, light response, florigen production and response, Abstract. We present BUSTED, a novel approach to identify gene-wide evidence of episodic positive selection, where the nonsynonymous substitution rate is transiently higher than the synonymous rate. BUSTED can be used on an entire phylogeny without requiring an a priori hypothesis regarding which branches lie beneath that branch. The candidate gene approach has been ubiquitously applied for genetic disease research, genetic association studies, biomarker and drug target selection in many organisms from animals to. To date, many candidate genes with economic characteristics or susceptibility to disease resistance have been detected primarily or even repeatedly, although there are currently no genetic signatures to predict disease relapse and survival. early stage cervical cancer. In this study, we investigate whether gene expression profiling of cervical cancer could be used to predict patient prognosis. A series of patients with primary cervical cancer who underwent radical hysterectomy between January, SPL transcription factors regulate important processes such as plant growth and development, metabolic regulation and abiotic stress . They play a crucial role in the development of floral organs. However, little is known about the characteristics and functions of SPLs in Orchidaceae. In this study, Cymbidium goeringii Rchb. F.